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Journal of the South African Veterinary Association

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Volume 71, Issue 2, June 2000

The fourteen editors of the Journal of the South African Veterinary Association: 1927-2000 : information
Bigalke, R.D.
68-76

Abstract: The initial motivation for having an article on the editors of the journal published in the year 2000 was to commemorate the 70 years of the existence of the Journal of the South African Veterinary Association. However, in conducting the research required to write the article it was established that the journal dates from 1927. Consequently the journal was about 73 rather than 70 years old at the end of 1999. During this period it has been faithfully served by 14 editors. This article contains a relatively brief description of the life histories, in relation to their terms of office as editors, of these editors, of whom 4 are still alive and actively pursuing their respective careers

Canine ehrlichioses : an update : review article
Kelly, P.J.
77-86

Abstract: The development of molecular biology techniques and methods for the isolation and growth of ehrlichias in tissue culture have greatly facilitated the study of these organisms. The available knowledge on ehrlichias is thus rapidly increasing and in this review recent findings on the epidemiology, transmission, clinical and laboratory signs of infection, diagnosis and treatment of canine ehrlichioses are described.

Use of herbal remedies by small-scale farmers to treat livestock diseases in central Eastern Cape Province, South Africa : article
Masika, P.J.Van Averbeke, W.Sonandi, A.
87-91

Abstract: The use of herbal remedies by African farmers in the treatment of livestock diseases was investigated using a range of methods, including group interviews, structured interviews, semi-structured interviews and participant observation. The study was concerned mainly with the `why' and `how' of herbal remedy use among African livestock holders farming on communally-owned land in central Eastern Cape Province. We found that the use of herbal remedies was still widespread because these remedies were cheap, locally available and convenient to administer. Farmers also used herbal remedies because they did not have knowledge of conventional remedies or found these too expensive and therefore used herbal remedies as a last resort. Most of the farmers interviewed (73 %) had used some form of herbal remedy. A small number of farmers did not use herbal remedies at all. They rejected them because they had bad experiences in the past or because they considered the practice outdated and socially inferior. Often farmers used herbal remedies in combination with conventional ones, but a substantial number relied on herbal remedies only. Farmers obtained herbal remedies principally by preparing their own or by purchasing from herbalists. Different plant parts were used to prepare herbal remedies in the form of infusions, decoctions, powders, pastes, and juices from fresh plant material. Preparations were made from a single plant or from a range of plants. Application of a remedy was by different routes and methods, depending on the perceived cause of the disease condition. Topical applications were used for skin conditions, powders were rubbed into incisions, drenches were common in the treatment of systemic conditions, and drops to treat ears and eyes. We identified several aspects of herbal remedy utilisation that could be subject to improvement, which included the absence of standard dose regimes, the potential toxicity of certain compounds present in plants used, over-exploitation of the plant resource and a lack of interest by the youth to learn the art.

Animal husbandry in Moretele 1 of North-West Province: implications for veterinary training and research : article
Letsoalo, S.S.Krecek, R.C.Botha, C.A.J.Ngetu, X.
92-96

Abstract: Little is known regarding the keeping of animals in the Moretele 1 area of North-West Province, South Africa. Therefore, the status and dynamics of animal husbandry, as well as a general assessment of the needs of animal owners in this area were researched. Results of the investigation will be used to make recommendations for improved veterinary extension servicing in the area. Semi-structured interviews, based on discussions with relevant stakeholders in the community and a resultant problem conceptualisation, were undertaken at 266 randomly selected households in 51 villages and centres in the area, after which the data was checked and verified before being captured and analysed. The findings reveal that within the field of veterinary extension delivery: 1) there is a demand for visual and written extension material, 2) the extension services must function where clients reside, 3) limitations in terms of infrastructure are present and should be addressed through partnerships and coordination amongst all the role-players in the Moretele 1 area, and 4) cattle and poultry are the most important of the animal species and should be the focus points of extension, but the need to curb zoonotic disease should not be disregarded. In this regard veterinary clinics, private veterinarians and other role-players should be used in partnership with extension workers. Lastly, the veterinary clinic is regarded as helpful in many respects by the community consulted and the service should be upgraded and made available to a wider client base, especially where private and state veterinarians are unavailable or too expensive in such resource-limited communities.

Ultrastructural characteristics of ostrich eggshell: outer shell membrane and the calcified layers : article
Richards, P.D.G.Richards, P.A.Lee, M.E.
97-102

Abstract: The ultrastructure of the eggshell of the domestic hen has been well researched and structural studies of other avian species, such as the ostrich, often base their interpretation of egg shell structure on that of the chicken. In the ostrich, lowered hatchability and hatching trauma may be due to shell ultrastructural abnormalities. In the present study the ultrastructure of the calcified portion, and the outer shell membrane (OSM), of domesticated ostrich eggshells was investigated using standard electron microscopic techniques. Transmission and scanning electron microscopy studies demonstrated intimate contact between cup-shaped structures present on the OSMand the mammillary layer of the calcified portion of the shell. The initial calcium carbonate growth of the calcified shell was of a dendritic nature with nucleation sites on the surface of the cup's contents. The dendritic growth gave way to a more randomly-orientated, smaller crystallite growth structure, which changed in formas it neared the vertical crystal layer (VCL). The VCL is described as being both amorphous and `crumbly' depending on the plane of fracture. These observations suggest that firstly, initial calcification is contained within the cups and is then directed outwards to formthe shell and that secondly, the VCL may contain an evolutionary, calcified cuticular layer. These observations serve as a baseline for studies investigating the effect of shell structure and strength on hatchling trauma and the influence of maternal diet.

An appropriate method for extracting the insect repellent citronellol from an indigenous plant (Pelargonium graveolens L'Her) for potential use by resource-limited animal owners : article
Botha, B.M.McCrindle, C.M.E.
103-105

Abstract: Veterinary needs appraisals in rural, peri-urban and urban areas have indicated a need for affordable and accessible veterinary health care. It was also found that farmers and animal owners used indigenous plants for treating animals. In Africa, insects such as Culex, Culicoides and Stomoxys maytransmit diseases, cause irritation to animals or prevent wound healing. Insect repellents used topically are generally safer and cheaper than insecticides. Using readily available commercial sources of ethanol 43 %v/v (brandy and cane spirits), it was shown that citronellol could be extracted from uncrushed leaves of the indigenous shrub Pelargonium graveolens L'Hér. Efficacy of extraction was compared to that using reagent grade absolute ethanol. The peak concentration of citronellol was achieved within 7 days of extraction and thereafter remained constant for 4 months. Extraction methods using tap water and cooking oil were not successful. The extraction was also less successful when the leaves were crushed or macerated before being placed into ethanol. Gas chromatography was used to monitor the concentration of citronellol in the different extracts.

Coccidian oocyst and nematode egg counts of free-ranging African buffalo (Syncerus caffer) in the Kruger National Park, South Africa : research communication
Penzhorn, B.L.
106-108

Abstract: Faecal specimens collected in the Kruger National Park from 103 African buffaloes (Syncerus caffer) up to 1 year old and 283 buffaloes older than 1 year were examined for the presence of coccidian oocysts and nematode eggs. Most specimens from animals older than 1 year had negative coccidian oocyst counts. Positive specimens from younger animals had significantly higher coccidian oocyst counts than those from older animals. No such difference was found for nematode egg counts.

Efficacy of a commercially available coryza vaccine against challenge with recent South African NAD-independent isolates of Haemophilus paragallinarum in chickens : research communication
Jacobs, A.A.C.Van der Werf, J.
109-110

Abstract: In South Africa the incidence of NAD-independent Haemophilus paragallinarum isolation from clinical cases is increasing. This study was carried out to test whether a commercially available coryza vaccine (Nobilis ® Coryza, Intervet International BV) could protect chickens against challenge with recent NAD-independent isolates. SPF chickens were vaccinated twice at 3 and 7 weeks of age and were challenged at 9 weeks of age with 5 different NAD-independent isolates of serotype A or C-3. The results after challenge show that the coryza vaccine induces good protection against challenge with the different South African NAD-independent isolates of H. paragallinarum, including serotype C-3.

Evaluation of two PCR-based procedures for typing Clostridium perfringens : research communication
Dungu, B.Henton, M.M.Bosman, A.Fourie, H.Viljoen, G.
111-114

Abstract: Two polymerase chain reaction (PCR)-based procedures for typing Clostridium perfringens, which affects most domestic animals, were compared and evaluated for efficiency as substitute to the guinea-pig intradermal test routinely used in our laboratory, namely a multiplex PCR and a protocol based on the individual amplification of gene sequences specific for each toxin. Reference isolates of C. perfringens types A, B, C and D as well as cultures from clinical specimens were tested. The sensitivity and specificity of the PCR was confirmed on reference isolates. There was similarity in results on 43 of the 46 samples typed by all 3 methods. Clear results were obtained by PCR on 5 clinical samples that showed either equivocal or weak skin reactions in guinea-pigs. The multiplex PCR protocol, in combination with the evaluation of bacterial growth, is a better alternative to in vivo toxin typing, since C. perfringens can only be incriminated as cause of a disease when it is present in large numbers in the intestine.

A retrospective study on some reproductive parameters of German shepherd bitches in Kenya : research communication
Mutembei, H.M.Mutiga, E.R.Tsuma, V.T.
115-117

Abstract: Data relating to reproductive parameters of German shepherd bitches were collected from registered German shepherd dog (GSD) breeders with information kept over a 15-year period (1982-1997). The information obtained was verified using the East African Kennel Club records. A total of 594 bitches from 280 breeders were recorded. >From these, 798 heats were observed, 594 of which were used for breeding, resulting in 3592 puppies. The mean age at puberty was 519.0 ± 41 days. Heats occurred throughout the year, although significantly (P < 0.05) higher and lower incidences were observed in October and April respectively. Pregnancy significantly (P < 0.01) increased interoestrous interval, which was 247.8 ± 99.6 and 183 ± 52 days among bred/pregnant and non-bred bitches respectively. Most bitches in oestrus (73.7 %) were bred, and breeding was carried out throughout the year, with a distribution closely related to that of heat incidence. Subsequently, whelping occurred throughout the year, and 95.5 %of the bitches that were mated whelped. A mean gestation period of 60.6 ± 5.1 days was observed. The mean litter size was 6.4 ± 0.4 puppies, and did not differ significantly between months. The preweaning losses were low, with 2.3 % stillbirths, 0.9 % culls and 11.4 % mortalities.

Von Willebrand's disease in the German shepherd dog : clinical communication
Lobetti, R.G.Dippenaar, T.
118-121

Abstract: Two litters of Germanshepherd dogs were evaluated for a haemorrhagic tendency that was characterised by excessive bleeding from the umbilicus at birth, haemorrhage and haematoma formation at vaccination, excessive bruising, and lameness due to haemarthrosis. Platelet counts, clotting times and Von Willebrand's factor (VWF) assays were assessed in all dogs. Factor VIII determination was performed in 1 puppy and its parents. Based on the clotting times and VWF assay, 6 puppies (4 male and 2 female) showed type I Von Willebrand's disease (VWD), 5 (4 male and 1 female) possible type II VWD, and 4 were unaffected. One puppy with possible type II VWD had very low factor VIII activity; its sire had a normal factor activity, whereas the dam was in the low-normal range. This article reports type I and possible type II VWD in 2 related litters of German shepherd dogs, the latter being rare in German shepherd dogs.

Progressive atrophic rhinitis in a medium-scale pig farm in Kiambu, Kenya : case report
Wabacha, J.K.Maribei, J.M.Thaiya, A.G.Munyua, S.J.M.Karanja, D.N.Njoroge, E.M.
122-124

Abstract: Forty-two pigs in a herd of 117 displayed various clinical signs of progressive atrophic rhinitis. The main signs included sneezing, coughing, lachrymation, serous to muco-purulent nasal discharge, and nasal bleeding in 1 pig. Three pigs had lateral deviation of the snout, while 4 had brachygnathia superior with obvious deformation of the face. Four acutely affected weaner pigs appeared weak, while the 7 chronically-affected pigs appeared smaller than their apparently unaffected penmates of the same age. Treatment of the acutely affected pigs with long-acting oxytetracycline at 20 mg/kg body weight intra-muscularly, repeated once after 7 days, reduced the severity but did not clear the sneezing from all the pigs. Fifteen pigs were slaughtered 2 months after the clinical diagnosis was made. The carcasses of the chronically affected pigs were about 15 % lighter than those of the apparently normal pigs of the same age and from the same pen, which translated to a loss of 921.00 Kenya shillings per pig (US$13.7). Diagnosis of progressive atrophic rhinitis was confirmed by sectioning the snouts of randomly selected slaughtered pigs with obvious deformation of the snout. Sections were madeat the level of the 1st/2nd upper premolar tooth. Varying degrees of turbinate atrophy, from mild to complete, were noted. Histopathology of the turbinates revealed metaplasia of nasal epithelium and fibrosis in the lamina propria.

Arrhythmogenic right ventricular cardiomyopathy in a dog : case report
Mohr, A.J.Kirberger, R.M.
125-130

Abstract: An 8-month-old Labrador retriever bitch was evaluated for sudden-onset, progressive abdominal distension. Physical examination revealed an exaggerated inspiratory effort, severe ascites, bilateral jugular vein distension, and hypokinetic femoral arterial pulses. Thoracic auscultation detected tachycardia with muffled heart sounds, without audible cardiac murmurs. Thoracic radiographs identified severe right ventricular enlargement and pleural effusion. The electrocardiogram was consistent with incomplete right bundle branch block or right ventricular enlargement. Echocardiography demonstrated severe right ventricular and atrial dilation, secondary tricuspid regurgitation, and thinning and hypocontractility of the right ventricular myocardium. Left heart chamber sizes were slightly decreased, with normal left ventricular contractility. Adiagnosis of arrhythmogenic right ventricular cardiomyopathy was reached, based on the characteristic clinical, electrocardiographic, radiographic and echocardiographic findings, and the exclusion of other causes of isolated right ventricular failure. Treatment effected good control of clinical signs, until acutely decompensated congestive right heart failure led to euthanasia after 4 months. Arrhythmogenic right ventricular cardiomyopathy is a well-described clinical entity in humans, and has previously been documented in 3 male dogs. The condition is characterised by progressive fibro-adipose replacement of right ventricular myocardium, while the left ventricle usually remains unaffected. It should be considered a differential diagnosis in any young dog presented with isolated right heart failure, syncope, or unexplained ventricular tachyarrhythmias. This article reports the 1st case of arrhythmogenic right ventricular cardiomyopathy in a female dog, and highlights its echocardiographic features.