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Journal of the South African Veterinary Association

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Volume 72, Issue 2, June 2001

Prevalence of Culicoides imicola and other species (Diptera: Ceratopogonidae) ateight sites in Zimbabwe : to the editor
Musuka, G.N.Mellor, P.S.Meiswinkel, R.Baylis, M.Kelly, P.J.
62-63

Abstract: Presents some prevalence data on Culicoides imicola and Culicoides bolitinos in Zimbabwe. Includes data on other common species, as revealed by collections made over 2 years at 8 widely-separated study sites

Chlamydial abortion in goats in the Umzimkulu District of Eastern Cape : to the editor
Jeyakumar, C.S.
63

Abstract: Reports on the prevalence of chlamydial abortion in goats in the Bossenfontein and Ngozi administrative areas in the Eastern Cape Province

The reversal of xylazine hydrochloride by yohimbine and 4-aminopyridine in goats : article
Ndeereh, D.R.Kihurani, D.O.Mbithi, P.M.F.
64-67

Abstract: Yohimbine, 4-aminopyridine, and a combination of the 2 drugs were studied to assess their potential as antagonists to xylazine in goats. Twenty-four small East African goats were divided randomly into 4 groups of 6 goats each in a placebo-controlled study. They were all treated with intramuscular xylazine at 0.44 mg/kg. At the time of maximum sedation, sterile water was administered intravenously to the control group, 0.15% 4-aminopyridine at 0.4mg/kg to Group 2, 0.1% yohimbine at 0.25 mg/kg to Group 3, and the combination of the 2 drugs at the same dose rates to Group 4. The yohimbine/4-amino-pyridine combination was also used to antagonise xylazine at 0.88mg/kg in 6 goats. The heart rate, respiratory rate and rate of ruminal movements, the pedal and palpebral reflexes as well as the reaction to noxious stimuli, the standing time and the total recovery time were established and evaluated to assess the effects of the treatments. The drugs reversed the xylazine-induced decrease in the heart rate, respiratory rate and rate of ruminal movements, and also rapidly restored the reflexes as well as the reaction to noxious stimulation. In addition, they significantly (P <0.05) decreased the mean standing time. The mean total recovery time was decreased significantly (P < 0.05) by 4-aminopyridine and the yohimbine/4-aminopyridine combination, but non-significantly (P > 0.05) by yohimbine. No relapse in sedation occurred. Overall, the combination of yohimbine and 4-aminopyridine produced better responses than the individual drugs, and may therefore be used for rapid reversal of xylazine-induced sedation in goats. Yohimbine or 4-aminopyridine may also be useful for this purpose but recovery may be prolonged.

Lumpy skin disease in southern Africa : a review of the disease and aspects of control : article
Hunter, P.Wallace, D.
68-71

Abstract: This article reviews some of the important aspects of lumpy skin disease (LSD) that may impact on its successful control. A resurgence of the disease in the last decade has highlighted some constraints of the Neethling strain vaccine, but there is no evidence of vaccine breakdowns owing to the presence of heterologous field strains. More research is needed on epidemiology and transmission of LSD in South Africa to formulate control measures.

Birth of live calves by in vitro embryo production of slaughtered cows in a commercial herd in South Africa : article
Arlotto, T.Gerber, D.Terblanche, S.J.Larsen, J.
72-75

Abstract: In vitro fertilisation (IVF) has become a useful breeding tool in most of the developed world. In this paper the success of bovine IVF and the birth of live calves under typical South African conditions is reported. Oocytes for IVF were collected from the ovaries of 6 slaughtered Bovelder beef cows. On average, 36.2 oocytes per donor were retrieved. From these oocytes, 43 blastocysts were produced from 5 of the donors by IVF with frozen Bovelder semen. The best 11 of these embryos were transferred into oestrous, synchronised Bovelder recipients in the same herd. As a result, 7 calves were born (a 64 %calving rate) from 4 of the original donors. The calves had a normal birth mass, but the mean gestation length of the male calves was significantly longer than the herd average (291.6 versus 285.2 days respectively). No calving difficulties were encountered. In summary, it was shown that IVF for bovine embryo production and transfer is possible on a commercial basis in South Africa.

Effects on anionic salts in a pre-partum dairy ration on calcium metabolism : article
Van Dijk, C.J.Lourens, D.C.
76-80

Abstract: The effects of anionic salts in the transition diet on serum and urine calcium at calving and on peripartal health, subsequent milk production and fertility performance were studied in a well-managed, high-producing Friesland dairy herd. Over a period of a year, approximately 21 days before the expected date of calving, 28 pre-partum heifers and 44 multiparous dry cows were randomly allocated within parity to 1 of 2 transition diets, designated control and experimental anionic diets. The anionic diet contained the same quantities of the basic transition ration fed to the control group as well as a standard anionic salt mixture containing 118 g NH4Cl, 36 g (NH4)2SO4 and 68 g MgSO4 (total 222 g) per animal per day. This reduced the DCAD to -11.68 mEq/100 g dietary dry matter compared to +13.57 for the control diet. Blood and urine were randomly sampled from 7 to 8 animals within each category within 3 hours post-partum. Serum calcium (total and ionised) and creatinine, urine calcium and creatinine and the fractional clearance of calcium were assessed. Relevant clinical, milk production, and fertility data were collected. The total serum calcium (2.07 versus 1.60 mmol/ ), serum ionised calcium (1.12 vs 1.02 mmol/ ), urine calcium (0.92 vs 0.10 mmol/ ) and the fractional clearance of calcium (1.88 vs 0.09 %) were significantly higher (P <0.01) at calving for multiparous cows fed the anionic diet compared to those fed the control diet. In the primiparous cows there were no significant differences in serumcalcium levels. However, the urine calcium (1.07 vs 0.43 mmol/ ) and the fractional clearance of calcium was higher (1.75 vs 0.45 %) in cows fed the anionic diet (P <0.05 and 0.01 respectively). These results illustrated that there were benefits, although no differences were demonstrated with respect to health, milk production or fertility. The supplementation of diets with anionic salts in the last 2-3 weeks before calving has the potential to significantly improve parturient calcium homeostasis.

Anaesthesia of gemsbok (Oryx gazella) with a combination of A3080, medetomidine and ketamine : article
Grobler, D.Bush, M.Jessup, D.Lance, W.
81-83

Abstract: An effective anaesthesia protocol was developed for adult free-ranging gemsbok (Oryx gazella) using a combination of A3080, medetomidine and ketamine. Ashort induction time; good muscle relaxation, adequate oxygenation and stable heart rate and respiration rate characterised this anaesthetic regime. Equal doses of A3080 and medetomidine (22-45 µg/kg) plus 200 mg of ketamine were administered to each animal. The anaesthesia was rapidly and completely reversed by intramuscular naltrexone at a dose of X = 0.9 ± 0.2 mg/kg and atipamezole at a dose X±90 ±20 µg/kg. No mortality or morbidity occurred with this protocol.

Analgesic and cardiopulmonary effects of intrathecally administered romifidine or romifidine and ketamine in goats (Capra hircus) : article
Aithal, H.P.AmarpalKinjavdekar, P.Pawde, A.M.Pratap, K.
84-91

Abstract: The study was conducted to evaluate the effects of romifidine alone (50 µg/kg) and a combination of romifidine (50 µg/kg) and ketamine (2.5 mg/kg) after intrathecal administration in goats. Ten adult goats of either sex weighing between 15 and 20 kg were randomly placed in 2 groups (groups I and II). The agents were administered at the lumbosacral subarachnoid space. Clinico-physiological parameters such as analgesia, motor incoordination, sedation, salivation, heart rate, respiratory rate, arterial pressure, central venous pressure and rectal temperature were studied. Other haematobiochemical parameters monitored were packed cell volume, haemoglobin, plasma proteins, glucose, urea and creatinine. The onset of analgesia was faster in group II (35.5 ±6.25 s) compared to that of group I (5.2 ±0.54 min). Analgesia of the tail, perineum, hind limbs, flank and thorax was mild to moderate in group I, but complete analgesia of tail, perineum and hind limbs was recorded in group II. Motor incoordination was mild in group I and severe in group II. Significant reduction in heart rate (more pronounced in group I) and respiratory rate (more pronounced in group II), and a significant increase in central venous pressure were recorded in both groups. Mean arterial pressure was reduced in both groups, but more markedly in group I. Sedation, electro-cardiogram, rectal temperature and haemato-biochemical parameters did not show significant differences between the 2 groups. The results of this study indicated a possible synergistic analgesic interaction between intrathecally administered romifidine and ketamine, without causing any marked systemic effects in goats.

Report of isolations of unusual lyssaviruses (rabies and Mokola virus) identified retrospectively from Zimbabwe : short communication
Bingham, J.Javangwe, S.Sabeta, C.T.Wandeler, A.I.Nel, L.H.
92-94

Abstract: Rabies isolates that had been stored between 1983 and 1997 were examined with a panel of anti-lyssavirus nucleocapsid monoclonal antibodies. Out of 56 isolates from cats and various wild carnivore species, 1 isolate of Mokola virus and 5 other non-typical rabies viruses were identified. The Mokola virus isolate was diagnosed as rabies in 1993 from a cat. Genetic analysis of this isolate suggests that it falls in a distinct subgroup of the Mokola virus genotype. The 5 non-typical rabies viruses were isolated from honey badgers (Mellivora capensis), African civets (Civettictis civetta) and an unidentified mongoose (Herpestidae). These isolates are representatives of rarely-reported wildlife-associated strains of rabies, probably maintained by the slender mongoose (Galerella sanguinea). These findings indicate that both Mokola virus and the mongoose-associated variant may be more common in Zimbabwe than is apparent from routine surveillance.

Isolation of Haemophilus somnus from dairy cattle in KwaZulu-Natal : an emerging cause of 'dirty cow syndrome' and infertility? : short communication
Last, R.D.Macfarlane, M.D.Jarvis, C.J.
95

Abstract: Haemophilus somnus was consistently isolated from vaginal discharges of dairy cows submitted from field cases of vaginitis, cervicitis and/or metritis in the KwaZulu-Natal Midlands during the period July 1995 - December 2000 and from the East Griqualand area in November/December 2000. The purulent vaginal discharges, red granular vaginitis and cervicitis, and pain on palpation described in these cases was very similar to that reported in outbreaks of H.somnus endometritis syndrome in Australia, Europe and North America. In all the herds involved in these outbreaks, natural breeding with bulls was employed. Although there was a good cure rate in clinically-affected animals treated with tetracyclines, culling rates for chronic infertility were unacceptably high. Employment of artificial insemination in these herds improved pregnancy rates in cows that had calved previously, but many cows that had formerly been infected failed to conceive.

Suspected post-vaccinal acute polyradiculoneuritis in a puppy : short communication
Gehring, R.Eggars, B.
96

Abstract: A 4-month-old German shepherd puppy developed hindquarter weakness after vaccination with a multivalent vaccine. This is suggestive of post-vaccinal polyradiculoneuritis. To date, only 1 similar case has been reported, which may be due to the under-reporting of suspected adverse drug reactions.

Prevalence of microorganisms associated with udder infections in dairy goats on small-scale farms in Kenya : short communication
Ndegwa, E.N.Munyua, S.J.M.Mulei, C. M.
97-98

Abstract: Six hundred and thirty clinically-normal milk samples from dairy goat flocks comprising a mixed population of German Alpine, Toggenburg, Saanen and Galla crosses were exam-ined over a 3-month period to determine the prevalence of bacterial organisms. Bacteria were isolated in 28.7 % of the milk samples (181/630) either singly (92.8 %) or in combination (7.2 %). The most prevalent bacterial organisms were Staphylococcus spp. (60.3 %), followed by Micrococcus spp. (17.7 %), Acinetobacter spp. (5 %), Actinomyces spp. (5%) and Streptococcus spp. (1.1 %). The Staphylococcus spp. were mainly coagulase negative (64.3 %). Coagulase-- negative staphylococci and coagulase-positive staphylococci accounted for 37.5 % and 22.7 % respectively of the total bacteria isolated. The isolation of bacteria, some of which are important in clinical and subclinical mastitis, in apparently normal caprine milk, indicates that particular attention should be given to the management of these dairy goat flocks in order to avoid the development of cases of clinical mastitis.

Temporary remission of disseminated paecilomycosis in a German shepherd dog treated with ketoconazole : case report
Booth, M.J.Van der Lugt, J.J.Van Heerden, A.Picard, J.A.
99-104

Abstract: Disseminated mycosis caused by Paecilomyces varioti in a female German shepherd dog presented with chronic forelimb lameness is described. Radiographs of the swollen carpal joint revealed geographic lysis of the radial epiphysis. Diagnosis was based on cytological demonstration of fungal hyphae and chlamydiospores, as well as fungal culture of fluid obtained by arthrocentesis. Temporary remission was characterised by markedly improved clinical signs and laboratory parameters, following treatment with ketoconazole. The dog was euthanased 9 months after the initial diagnosis, following the diagnosis of multifocal discospondylitis. This appears to be the longest described period of temporary remission obtained with treatment in dogs with paecilomycosis. Clinical, clinicopathological and necropsy findings of this disease in another German shepherd dog are briefly described.

First report of a parasitic mite, Leptotrombidium (Hypotrombidium) subquadratum (Lawrence) (Acari : Trombiculidae : Trombiculinae), from dogs and children in the Bloemfontein area, South Africa : case report
Heyne, H.Coetzee, L.
105-106

Abstract: Leptotrombidium subquadratum larvae were collected for the first time in 1994 from dogs in Bloemfontein. The larvae have been collected annually, during the summer months, over a period of 6-7 years. Previously the only known hosts were scrub hare (Lepus saxatilis) (locality unknown) and short-snouted elephant shrew (Elephantulus brachyrhynchus) (Kruger National Park). These mites cause severe itching and dermatitis in humans and dogs.

Parasitological Challenges 2000 and Beyond : Parasitological Society of Southern Africa : conference abstracts 109-116

Abstract: Abstracts of papers presented at the 28th Annual Scientific Meeting of the Parasitological Society of Southern Africa - Parasitological Challenges 2000 and Beyond - held from 27-29 September 2000 in Bloemfontein.